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Received Oct 30; Accepted Feb 5. Using simultaneous intracellular recordings from both the LM and CM layers during the peristaltic reflex, we have shown unequivocally that the two muscle layers of the distal colon receive simultaneous inhibitory neuromuscular inputs during descending inhibition, and simultaneous excitatory neuromuscular inputs during ascending excitation. Spontaneous intracellular electrical activity recorded simultaneously from the LM and CM In the LM, spontaneous rhythmic depolarizations are shown. During an episode from October he suggested that movies depicting the evils of slavery and segregation foment anti-white violence.

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In the current study, we have used simultaneous intracellular recording techniques from the LM and CM, to compare the nature of neuromuscular transmission to the two muscle layers of the colon during mucosal stimulation and balloon distension. During an episode from October he suggested that movies depicting the evils of slavery and segregation foment anti-white violence. Physiology of the enteric nervous system. It is noteworthy that in 2 of the 4 animals where atropine abolished the ascending excitatory reflex, it was possible to elicit IJPs ascending inhibition synchronously in the LM and CM Fig. Final images were constructed with BioRad Comos software. These action potentials gave rise to strong contractions which usually dislodged at least one of the microelectrodes.

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Jun 15,  · METHODS. Guinea-pigs weighing – g were killed by CO 2 inhalation overdose, in accordance with the animal ethics committee of the University of Nevada School of Medicine. The abdominal cavity was opened and the terminal 10–15 cm of distal colon was removed, the mesenteric attachment trimmed away, the lumen flushed clean with Krebs solution, and the colon placed Cited by: Jun 06,  · Fixed tissues were incubated overnight at 4 °C with respective primary antibodies to RECA-1 (Mouse monoclonal, Abcam, ) and CCL2 (Rabbit polyclonal, Abcam, ).

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